Three-Dimensional Loop Extrusion

TL;DR

This paper introduces a 3D 'trans-grabbing' model for DNA loop extrusion by SMC proteins, explaining experimental observations like Z-loop formation, bypassing roadblocks, and predicting new behaviors in DNA looping.

Contribution

The paper presents the first 3D model that reproduces experimental features of loop extrusion and predicts novel Z-loop formations and behaviors.

Findings

Reproduces experimental features of loop extrusion.

Predicts formation of Z-loops via multiple SMCs.

Suggests tethering influences Z-loop asymmetry.

Abstract

Loop extrusion convincingly describes how certain Structural Maintenance of Chromosome (SMC) proteins mediate the formation of large DNA loops. Yet, most of the existing computational models cannot reconcile recent in vitro observations showing that condensins can traverse each other, bypass large roadblocks and perform steps longer than its own size. To fill this gap, we propose a three-dimensional (3D) "trans-grabbing" model for loop extrusion which not only reproduces the experimental features of loop extrusion by one SMC complex, but also predicts the formation of so-called "Z-loops" via the interaction of two or more SMCs extruding along the same DNA substrate. By performing Molecular Dynamics simulations of this model we discover that the experimentally observed asymmetry in the different types of Z-loops is a natural consequence of the DNA tethering in vitro. Intriguingly, our model predicts this bias to disappear in absence of tethering and a third type of Z-loop, which has not yet been identified in experiments, to appear. Our model naturally explains road-block bypassing and the appearance of steps larger than the SMC size as a consequence of non-contiguous DNA grabbing. Finally, it is the first to our knowledge to address how Z-loops and bypassing might occur in a way that is broadly consistent with existing cis-only 1D loop extrusion models.