Scattering fingerprints of two-state dynamics

TL;DR

This paper introduces a theoretical framework for analyzing two-state particle dynamics using scattering fingerprints, aiding the interpretation of complex transport phenomena in biological and material systems.

Contribution

It develops a novel approach to identify multi-state dynamics through scattering signatures, complementing existing analysis methods.

Findings

Framework successfully applied to model systems and cell experiments

Enables differentiation between single- and multi-state transport

Applicable to various scattering techniques like DDM, neutron, and X-ray scattering

Abstract

Particle transport in complex environments such as the interior of living cells is often (transiently) non-Fickian or anomalous, that is, it deviates from the laws of Brownian motion. Such anomalies may be the result of small-scale spatio-temporal heterogeneities in, or viscoelastic properties of, the medium, molecular crowding, etc. Often the observed dynamics displays multi-state characteristics, i.e. distinct modes of transport dynamically interconverting between each other in a stochastic manner. Reliably distinguishing between single- and multi-state dynamics is challenging and requires a combination of distinct approaches. To complement the existing methods relying on the analysis of the particle's mean squared displacement, position- or displacement-autocorrelation function, and propagators, we here focus on "scattering fingerprints" of multi-state dynamics. We develop a theoretical framework for two-state scattering signatures -- the intermediate scattering function and dynamic structure factor -- and apply it to the analysis of simple model systems as well as particle-tracking experiments in living cells. We consider inert tracer-particle motion as well as systems with an internal structure and dynamics. Our results may generally be relevant for the interpretation of state-of-the-art differential dynamic microscopy experiments on complex particulate systems, as well as inelastic or quasielastic neutron (incl. spin-echo) and X-ray scattering scattering probing structural and dynamical properties of macromolecules, when the underlying dynamics displays two-state transport.