Using Experimentally Calibrated Regularized Stokeslets to Assess Bacterial Flagellar Motility Near a Surface

TL;DR

This study calibrates a numerical method for modeling bacterial flagellar motion near surfaces, validating it with experiments, and analyzes how morphology affects swimming efficiency and energy costs.

Contribution

It introduces experimentally calibrated regularized Stokeslets for accurate near-surface bacterial motility simulations, providing new insights into morphology effects on swimming performance.

Findings

Optimal regularization parameters achieved less than 5% difference with experiments.

All measures predicted the same optimal flagellar wavelength regardless of size and proximity.

Body size influences energy cost of bacterial motility near surfaces.

Abstract

The presence of a nearby boundary is likely to be important in the life cycle and evolution of motile flagellate bacteria. This has led many authors to employ numerical simulations to model near-surface bacterial motion and compute hydrodynamic boundary effects. A common choice has been the method of images for regularized Stokeslets (MIRS); however, the method requires discretization sizes and regularization parameters that are not specified by any theory. To determine appropriate regularization parameters for given discretization choices in MIRS, we conducted dynamically similar macroscopic experiments and fit the simulations to the data. In the experiments, we measured the torque on cylinders and helices of different wavelengths as they rotated in a viscous fluid at various distances to a boundary. We found that differences between experiments and optimized simulations were less than 5\% when using surface discretizations for cylinders and centerline discretizations for helices. Having determined optimal regularization parameters, we used MIRS to simulate an idealized free-swimming bacterium constructed of a cylindrical cell body and a helical flagellum moving near a boundary. We assessed the swimming performance of many bacterial morphologies by computing swimming speed, motor rotation rate, Purcell's propulsive efficiency, energy cost per distance, and a new metabolic energy cost defined to be the energy cost per body mass per distance. All five measures predicted the same optimal flagellar wavelength independently of body size and surface proximity. Although the measures disagreed on the optimal body size, they all predicted that body size is an important factor in the energy cost of bacterial motility near and far from a surface.