Sickle Cell Disease Severity Prediction from Percoll Gradient Images using Graph Convolutional Networks

TL;DR

This paper introduces a novel computational method using graph convolutional networks and neural networks to predict sickle cell disease severity from Percoll gradient images, reducing reliance on expensive lab tests.

Contribution

It presents the first computational approach for SCD severity prediction that combines image analysis with deep learning, enabling cost-effective assessment.

Findings

Achieved prediction performance close to methods using ground-truth lab measurements.

Utilized inexpensive blood analysis tools for severity prediction.

Demonstrated effectiveness on a cohort of 216 subjects.

Abstract

Sickle cell disease (SCD) is a severe genetic hemoglobin disorder that results in premature destruction of red blood cells. Assessment of the severity of the disease is a challenging task in clinical routine since the causes of broad variance in SCD manifestation despite the common genetic cause remain unclear. Identification of the biomarkers that would predict the severity grade is of importance for prognosis and assessment of responsiveness of patients to therapy. Detection of the changes in red blood cell (RBC) density through separation of Percoll density gradient could be such marker as it allows to resolve intercellular differences and follow the most damaged dense cells prone to destruction and vaso-occlusion. Quantification of the images obtained from the distribution of RBCs in Percoll gradient and interpretation of the obtained is an important prerequisite for establishment of this approach. Here, we propose a novel approach combining a graph convolutional network, a convolutional neural network, fast Fourier transform, and recursive feature elimination to predict the severity of SCD directly from a Percoll image. Two important but expensive laboratory blood test parameters measurements are used for training the graph convolutional network. To make the model independent from such tests during prediction, the two parameters are estimated by a neural network from the Percoll image directly. On a cohort of 216 subjects, we achieve a prediction performance that is only slightly below an approach where the groundtruth laboratory measurements are used. Our proposed method is the first computational approach for the difficult task of SCD severity prediction. The two-step approach relies solely on inexpensive and simple blood analysis tools and can have a significant impact on the patients' survival in underdeveloped countries where access to medical instruments and doctors is limited