Diffusion time dependence, power-law scaling, and exchange in gray matter

TL;DR

This study investigates how diffusion MRI signals in gray matter are affected by water exchange, morphology, and microstructure, revealing a power-law signature and exchange effects that improve understanding of tissue microstructure.

Contribution

The paper demonstrates through simulations and experiments that exchange influences diffusion signals in gray matter and identifies conditions where neurite signals dominate, advancing biophysical modeling accuracy.

Findings

Observed GM stick power-law consistent with impermeable neurites

Detected significant water exchange in gray matter

Identified experimental regimes where neurite signals dominate

Abstract

Diffusion MRI (dMRI) provides contrast that reflect diffusing spins' interactions with microstructural features of biological systems, but its specificity remains limited due to the ambiguity of its relation to the underlying microstructure. To improve specificity, biophysical models of white matter (WM) typically express dMRI signals according to the Standard Model (SM) and have more recently in gray matter (GM) attempted to incorporate cell soma (the SANDI model). The validity of the assumptions underlying these models, however, remains largely undetermined, especially in GM. Observing the models' unique, functional properties, such as the $b^{-1/2}$ power-law associated with 1d diffusion, has emerged as a fruitful strategy for experimental validation. The absence of this signature in GM has been explained by neurite water exchange, non-linear morphology, and/or obscuring soma signal contributions. Here, we present simulations in realistic neurons demonstrating that curvature and branching does not destroy the stick power-law in impermeable neurites, but that their signal is drowned by the soma under typical experimental conditions: Nevertheless, we identify an attainable experimental regime in which the neurite signal dominates. Furthermore, we find that exchange-driven time dependence produces a behavior opposite to that expected from restricted diffusion, thereby providing a functional signature disambiguating the two effects. We present data from dMRI experiments in ex vivo rat brain at ultrahigh field and observe a time dependence consistent with substantial exchange and a GM stick power-law. The first finding suggests significant water exchange while the second suggests a small sub-population of impermeable neurites. To quantify our observations, we harness the K\"arger exchange model and incorporate the corresponding signal time dependence in SM and SANDI.