Macaque's Cortical Functional Connectivity Dynamics at the Onset of Propofol-Induced Anesthesia

TL;DR

This study analyzes macaque cortical connectivity dynamics during propofol-induced anesthesia, revealing how functional networks evolve in the seconds leading to loss of consciousness using time-resolved Granger causality analysis.

Contribution

It provides the first detailed description of cortical functional connectivity changes during the transition to anesthesia in macaques, highlighting specific regional interactions.

Findings

Connectivity increases 1 minute after propofol administration

Predominant flow from occipital/temporal to frontal/parietal regions

Impaired connectivity from frontal/parietal to occipital/temporal regions during anesthesia

Abstract

Propofol, when administered for general anesthesia, induces oscillatory dynamic brain states that are thought to underlie the drug's pharmacological effects. Despite the elucidation of propofol's mechanisms of action at the molecular level, its effects on neural circuits and overall cortical functioning, which eventually lead to unconsciousness, are still unclear. To identify possible mechanisms, the spatial-temporal patterns of functional connectivity established among specialized cortical areas in anesthetized subjects need to be described. Within this context, the present research involved the analysis of dense sub-dural ECoG electrode array recordings from macaques under propofol anesthetic induction. Granger causality methodology was used to infer functional connectivity interactions in five physiological frequency bands serially over time, every five seconds throughout the experiment. The time-resolved networks obtained permitted us to observe the unfolding of the anesthetic induction and compare the networks obtained under different experimental conditions. About one minute after administering propofol, functional connectivity started to increase for 4-5 minutes, then decreased until the LOC was achieved. A predominant Granger causality flow from the occipital and temporal areas towards the frontal and parietal regions was also evidenced during the transition. During general anesthesia, the local connectivity of the occipital lobe increased, as did the interactions between the occipital and temporal lobes. Conversely, the functional connectivity from the frontal and parietal lobes toward the temporal and occipital regions was mainly impaired. The research is one of the first studies to describe the dynamics of the functional connectivity during the transitional state that precedes the LOC.