Equilibrium Properties of E. coli Lactose Permease Symport -- A Random-walk Model Approach

TL;DR

This paper models the equilibrium properties of E. coli lactose-H+ symport using a novel cotransport model that accounts for leakage, analyzing how parameters affect equilibrium and stabilization time.

Contribution

Introduces a new cotransport model that includes leakage and satisfies static head equilibrium, providing insights into the dynamics of E. coli LacY symport.

Findings

Leakage does not affect equilibrium solution but shortens the time to reach equilibrium.

Equilibrium time increases with the periplasm to cytoplasm volume ratio.

Cell contraction prolongs the time for cytoplasm pH stabilization.

Abstract

The symport of lactose and H+ is an important physiological process in E. coli, for it is closely related to cellular energy supply. In this paper, the symport of H+ and lactose by E. coli LacY protein is computationally simulated using a newly proposed cotransport model that takes the "leakage" phenomenon (uncoupled sugar translocation) into account and also satisfies the static head equilibrium condition. Then, we study the equilibrium properties of this cotransport process including equilibrium solution and the time required to reach equilibrium state, when varying the parameters of the initial state of cotransport system. It can be found that in the presence of leakage, H+ and lactose will reach their equilibrium state separately, but the intensity of "leakage" has almost no effect on the equilibrium solution, while the stronger leakage is, the shorter the time required for H+ and lactose to reach equilibrium. For E. coli cells with different periplasm and cytoplasm volumes, when cotransport is performed at constant initial particle concentration, the time for cytoplasm pH to be stabilized increases monotonically with the periplasm to cytoplasm volume ratio. For a certain E. coli cell, as it continues to lose water and contract, the time for cytoplasm pH to be stabilized by cotransport also increases monotonically when the cell survives. The above phenomena and other findings in this paper may help us to not only further validate or improve the model, but also deepen our understanding of the cotransport process of E. coli LacY protein.