Disruptive effects of chlorpyrifos on predator-prey interactions of Ceratophrys ornata tadpoles: consequences at the population level using computational modeling

TL;DR

This study combines experimental ecotoxicology and computational modeling to assess how chlorpyrifos affects tadpole predator-prey interactions, revealing behavioral and population-level impacts and demonstrating the utility of modeling as a predictive tool.

Contribution

It introduces a novel integrated approach using experiments and computational models to analyze pesticide effects on amphibian behavior and interactions.

Findings

Chlorpyrifos causes mortality and behavioral changes in tadpoles.

Behavioral effects persist during predator-prey interactions.

Model predictions align with experimental data within errors.

Abstract

Large-scale ecotoxicological studies have technical and ethical limitations, both related to the need to expose individuals to potentially harmful compounds. Computational modeling is a complementary useful and predictive tool that overcomes these limitations. Considering the increasing interest in the effects of pesticides on behavioral traits, the aim of this study was to evaluate the effects of chlorpyrifos (CPF) on intra- and inter-specific interactions of anuran tadpoles, complementing traditional ecotoxicological tools with a theoretical analysis verified by computational simulations. Experiments were developed under two consecutive phases: a first phase of exposure, and a second phase of interactions. The second phase consisted of evaluating the effects of CPF on intra- and inter-specific interactions of exposed C. ornata (Co) tadpoles acting as predators and unexposed Rhinella fernandezae (Rf) tadpoles acting as prey, under different predator-prey proportions. Also, intraspecific interactions of three Co tadpoles under different conditions of exposure were evaluated. During the exposure phase, chlorpyrifos induced significant mortality from 48h, irregular swimming, tail flexure, and the presence of subcutaneous air. Also, it induced effects on the sounds emitted after 96h of exposure, registering less number of pulses and higher dominant frequencies, and altered intra- and inter-specific interactions. During the interaction phase, the larvae continued to show sound effects, however, the antipredator mechanism continued to be operating and efficient. Finally, it was possible to model the behavior of the larvae under the effects of chlorpyrifos. We conclude that experimental data and computational modeling matched within errors. Therefore, computational simulation is a valuable ecotoxicological tool that provides new information and allows predicting natural processes.