Rapid micro-immunohistochemistry
Robert D. Lovchik, David Taylor, Govind Kaigala
TL;DR
This paper introduces a microfluidic probe-based method for rapid, localized immunohistochemical staining of tissue sections, significantly reducing processing time and enabling precise protein detection.
Contribution
The authors developed a novel microfluidic probe system with tailored apertures for fast, localized IHC, improving speed and spatial control over traditional methods.
Findings
Enables rapid IHC within minutes
Provides localized staining with high spatial precision
Reduces reagent consumption and processing time
Abstract
We present a new and versatile implementation of rapid and localized immunohistochemical staining of tissue sections. Immunohistochemistry (IHC) allows to detect specific proteins on tissue sections and comprises a sequence of specific biochemical reactions. For the rapid implementation of IHC, we fabricated horizontally oriented microfluidic probes (MFP) with functionally designed apertures to enable square and circular footprints, which we employ to locally expose a tissue to time-optimized sequences of different biochemicals.
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