Induction of VEGF secretion from bone marrow stromal cell line (ST-2) by the dissolution products of mesoporous silica glass particles containing CuO and SrO

TL;DR

This study investigates how dissolution products from specially designed mesoporous silica glasses containing CuO and SrO can stimulate VEGF secretion from bone marrow stromal cells, potentially aiding in vascularized bone tissue engineering.

Contribution

It introduces new bioactive glass compositions containing CuO and SrO that enhance VEGF secretion without cytotoxic effects, advancing bone regeneration strategies.

Findings

CuSr-1 and CuSr-2.5 glasses increased VEGF secretion after 48 hours

Dissolution products of these glasses are non-cytotoxic to ST-2 cells

Combination of Cu2+ and Sr2+ ions promotes angiogenic factor secretion

Abstract

Certain biomaterials are capable of inducing the secretion of Vascular Endothelial Growth Factor (VEGF) from cells exposed to their biochemical influence, which plays a vital role in stimulating angiogenesis. Looking for this capacity, in this study three porous glasses were synthesized and characterized. The objective of this study was to determine the concentration of the glass particles that, being out of the cytotoxic range, could increase VEGF secretion. The viability of cultivated bone marrow stromal cells (ST-2) was assessed. The samples were examined with light microscopy (LM) after the histochemical staining for haematoxylin and eosin (HE). The biological activity of glasses was evaluated in terms of the influence of the Cu2+ and Sr2+ ions on the cells. The dissolution products of CuSr-1 and CuSr-2.5 produced the highest secretion of VEGF from ST-2 cells after 48 h of incubation. The combination of Cu2+ and Sr2+ lays the foundation for engineering a bioactive glass than can lead to vascularized, functional bone tissue when used in bone regeneration applications.