Multiplicative noise underlies Taylor's law in protein concentration fluctuations in single cells

TL;DR

This study demonstrates that protein concentration fluctuations in single cells follow Taylor's law due to multiplicative noise, supported by experiments, modeling, and analytical solutions, revealing noise in production rates as the dominant factor.

Contribution

The paper introduces a mechanistic model and analytical framework showing that multiplicative noise explains Taylor's law in protein concentration fluctuations in single cells.

Findings

Protein fluctuations follow a square power-law dependence on the mean.

Model reproduces experimental distributions across conditions.

Noise in production rates dominates over division noise.

Abstract

Protein concentration in a living cell fluctuates over time due to noise in growth and division processes. From extensive single-cell experiments by using E. coli strains with different promoter strength (over two orders of magnitude) and under different nutrient conditions, we found that the variance of protein concentration fluctuations follows a robust square power-law dependence on its mean, which belongs to a general phenomenon called Taylor's law. To understand the mechanistic origin of this observation, we use a minimal mechanistic model to describe the stochastic growth and division processes in a single cell with a feedback mechanism for regulating cell division. The model reproduces the observed Taylor's law. The predicted protein concentration distributions agree quantitatively with those measured in experiments for different nutrient conditions and a wide range of promoter strength. By using a mean-field approximation, we derived a single Langevin equation for protein concentration with multiplicative noise, which can be solved exactly to prove the square Taylor's law and to obtain an analytical solution for the protein concentration distribution function that agrees with experiments. Analysis of experiments by using our model showed that noise in production rates dominates over noise from cell division in contributing to protein concentration fluctuations. In general, a multiplicative noise in the underlying stochastic dynamics may be responsible for Taylor's law in other systems.