Effect of Solution and Post-Mortem Time on Mechanical and Histological Properties of Liver during Cold Preservation

TL;DR

This study investigates how different preservation solutions and time durations affect the mechanical and histological properties of bovine liver tissue during cold storage, providing insights into optimal preservation methods.

Contribution

It introduces a combined mechanical and histological analysis of liver preservation, highlighting the impact of solution type and preservation duration on tissue properties.

Findings

Preservation solution and time significantly affect liver tissue properties.

Mechanical properties vary with different preservation solutions.

Histological integrity declines over time during preservation.

Abstract

In this study, we examine the mechanical and histological properties of the bovine liver tissue stored in Lactated Ringers (control), HTK and UW solutions as a function of preservation period. The mechanical experiments are conducted with a shear rheometer on cylindrical tissue samples extracted from 3 bovine livers and the change in viscoelastic material properties of the bovine liver is characterized using the fractional derivative Kelvin-Voigt Model. Also, the histological examinations are performed on the same liver samples under a light microscope. The results show that the preservation solution and period have a significant effect on the mechanical and histological properties of the liver tissue.