Hyperspectral Absorption Microscopy Using Photoacoustic Remote Sensing

TL;DR

This paper presents an advanced hyperspectral photoacoustic remote sensing method for non-destructive optical absorption imaging, capable of detecting various endogenous chromophores in biological tissues with high sensitivity.

Contribution

It introduces a tunable nanosecond light source for wide-range excitation and demonstrates the technique's effectiveness in biological tissue analysis and cellular imaging.

Findings

Successful characterization of water infrared absorption spectrum

Recovery of cell nuclei and oxygen saturation in chicken embryo

Identification of adipocytes in murine adipose tissue

Abstract

An improved method of remote optical absorption spectroscopy and hyperspectral optical absorption imaging is described which takes advantage of the photoacoustic remote sensing detection architecture. A wide range of photoacoustic excitation wavelengths ranging from 210 nm to 1550 nm was provided by a nanosecond tunable source allowing access to various salient endogenous chromophores such as DNA, hemeproteins, and lipids. Sensitivity of the device was demonstrated by characterizing the infrared absorption spectrum of water. Meanwhile, the efficacy of the technique was explored by recovering cell nuclei and oxygen saturation from a live chicken embryo model and by recovering adipocytes from freshly resected murine adipose tissue. This represents a continued investigation into the characteristics of the hyperspectral photoacoustic remote sensing technique which may represent an effective means of non-destructive endogenous contrast characterization and visualization.