Fast ATP-dependent Subunit Rotation in Reconstituted FoF1-ATP Synthase Trapped in Solution

TL;DR

This study uses an advanced trapping technique to observe and analyze the rapid, fluctuating subunit rotation in reconstituted FoF1-ATP synthases, revealing new insights into their kinetic behavior and maximum speeds.

Contribution

The paper demonstrates prolonged single-molecule observation of FoF1-ATP synthase rotation using an ABEL trap, enabling detailed kinetic analysis in solution.

Findings

Fluctuating ATP-dependent catalytic rates observed in individual enzymes.

Electrochemical potential limits maximum ATP hydrolysis rate.

Maximum rotation speed measured was 180 rounds/sec, faster than ensemble averages.

Abstract

FoF1-ATP synthases are ubiquitous membrane-bound, rotary motor enzymes that can catalyze ATP synthesis and hydrolysis. Their enzyme kinetics are controlled by internal subunit rotation, by substrate and product concentrations, by mechanical inhibitory mechanisms, but also by the electrochemical potential of protons across the membrane. Single-molecule F\"orster resonance energy transfer (smFRET) has been used to detect subunit rotation within FoF1-ATP synthases embedded in freely diffusing liposomes. We now report that kinetic monitoring of functional rotation can be prolonged from milliseconds to seconds by utilizing an Anti-Brownian electrokinetic trap (ABEL trap). These extended observation times allowed us to observe fluctuating rates of functional rotation for individual FoF1-liposomes in solution. Broad distributions of ATP-dependent catalytic rates were revealed. The buildup of an electrochemical potential of protons was confirmed to limit the maximum rate of ATP hydrolysis. In the presence of ionophores or uncouplers, the fastest subunit rotation speeds measured in single reconstituted FoF1-ATP synthases were 180 full rounds per second. This was much faster than measured by biochemical ensemble averaging, but not as fast as the maximum rotational speed reported previously for isolated single F1 complexes uncoupled from the membrane-embedded Fo complex. Further application of ABEL trap measurements should help resolve the mechanistic causes of such fluctuating rates of subunit rotation.