Super-resolution structured illumination microscopy: past, present and future
Kirti Prakash, Benedict Diederich, Stefanie Reichelt, Rainer, Heintzmann, Lothar Schermelleh
TL;DR
This paper reviews the evolution, current state, and future directions of structured illumination microscopy (SIM), highlighting technological advances, applications, and related super-resolution techniques in biological imaging.
Contribution
It provides a comprehensive overview of SIM's development, including hardware, software, and biological applications, and discusses future trends and related imaging methods.
Findings
Recent SIM technological advancements
Integration of SIM with computational imaging
Emerging trends in super-resolution microscopy
Abstract
Structured illumination microscopy (SIM) has emerged as an essential technique for 3D and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the various aspects of SIM, from bespoke hardware and software development and the use of commercial instruments to biological applications. This special issue aims to recap recent developments as well as outline future trends. In addition to SIM, we cover related topics such as complementary super-resolution microscopy techniques, computational imaging, visualisation and image processing methods.
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