Evidence for microscopic kurtosis in neural tissue revealed by Correlation Tensor MRI

TL;DR

This study uses Correlation Tensor MRI to detect microscopic kurtosis in vivo in rat brains, revealing its significant contribution to diffusion MRI signals and highlighting its importance as a potential biomarker.

Contribution

The paper introduces an improved, faster CTI protocol for robust in vivo estimation of microscopic kurtosis, demonstrating its significance in healthy brain tissue.

Findings

Positive microscopic kurtosis detected in white and grey matter.

Microscopic kurtosis is the dominant kurtosis source in grey matter.

Neglecting $K$ biases prior diffusion kurtosis analyses.

Abstract

Purpose: The impact of microscopic diffusional kurtosis ($\mu K$) - arising from restricted diffusion and/or structural disorder - remains a controversial issue in contemporary diffusion MRI (dMRI). Recently, Correlation Tensor MRI (CTI) was introduced to disentangle the sources contributing to diffusional kurtosis, without relying on a-priori assumptions. Here, we aimed to investigate $\mu K$ in in vivo rat brains and assess its impact on state-of-the-art methods ignoring $\mu K$. Methods: CTI harnesses double diffusion encoding (DDE) experiments, which were here improved for speed and minimal bias using four different sets of acquisition parameters. The robustness of CTI estimates from the improved protocol is assessed in simulations. The in vivo CTI acquisitions were performed in healthy rat brains using a 9.4T pre-clinical scanner equipped with a cryogenic coil, and targeted the estimation of $\mu K$, anisotropic kurtosis, and isotropic kurtosis. Results: The improved CTI acquisition scheme substantially reduces scan time and importantly, also minimizes higher-order-term biases, thus enabling robust $\mu K$ estimation, alongside Kaniso and Kiso metrics. Our CTI experiments revealed positive $\mu K$ both in white and grey matter of the rat brain in vivo; $\mu K$ is the dominant kurtosis source in healthy grey matter tissue. The non-negligible $\mu K$ substantially biases prior state-of-the-art analyses of Kiso and Kaniso. Conclusion: Correlation Tensor MRI offers a more accurate and robust characterization of kurtosis sources than its predecessors. $\mu K$ is non-negligible in vivo in healthy white and grey matter tissues and could be an important biomarker for future studies. Our findings thus have both theoretical and practical implications for future experiments.