Detecting cell and protein concentrations by the use of a thermal based sensor

TL;DR

This paper explores a thermal sensor based on the Transient Plane Source method to detect cell growth and protein concentrations by monitoring thermal properties, offering a receptor-free biosensing approach with promising initial results.

Contribution

The study develops and tests a thermal sensor technique for detecting biological concentrations without natural receptors, advancing biosensor technology.

Findings

Thermal properties vary with cell and protein concentrations.

Initial tests show promise for cell detection applications.

Further research needed for sensitivity and applicability.

Abstract

Biosensors are frequently used nowadays for the sake of their attractive capabilities. Because of their high accuracy and precision, they are more and more used in the medical sector. Natural receptors are mostly used, but their use have some specific drawbacks. Therefore, new read-out methods are being developed where there is no need for these receptors. Via a Transient Plane Source (TPS) sensor, the thermal properties of a fluid can be determined. These sensors can detect the capability of a fluid to absorb heat i.e. the thermal effusivity. By the way of monitoring this property, many potential bioprocesses can be monitored. The use of this promising technique was further developed in this research for later use of detecting cell growth and protein concentrations. Firstly, the thermal properties of growth medium and yeast cells were determined. Here, it became clear that the thermal properties change in different concentrations. Also, measurements were performed on protein concentration. No unambiguously results were obtained from these tests. But, the overall results from the use of this sensor are very promising, especially in the cell detection compartment. However, further research will tell about the applicability and sensitivity of this type of sensor.