Extraction, isolation, structure elucidation and evaluation of toxicity, anti-inflammatory and analgesic activity of Pituranthos scoparius constituents

TL;DR

This study investigates the traditional use, chemical constituents, toxicity, and anti-inflammatory and analgesic activities of Pituranthos scoparius, including compound isolation and activity evaluation.

Contribution

It is the first to isolate and characterize chemical constituents of P. scoparius and evaluate their biological activities and toxicity.

Findings

24.47% of people use the plant in folk medicine

Four compounds were isolated and characterized from the extract

The plant exhibits potential anti-inflammatory and analgesic effects

Abstract

The present work aimed to investigate an ethnobotanical survey about Pituranthos scoparius and assess the toxicity, anti-inflammatory (in vitro, and in vivo) potential, in vitro antioxidant, and analgesic effects of stems and roots of Pituranthos scoparius. Furthermore; to isolate and elucidate the chemical constituents of the n-butanol stem extract of P. scoparius (ButE) and determine the toxicity and anti-inflammatory effects of these compounds added to the ButE. Data from an ethnopharmacological study showed that 24.47 % of people used this plant in folk medicine. Four compounds were isolated from ButE. These compounds were characterized by means of NMR and high-resolution mass spectral (HRMS) data.