Finer-Focused Partial Wave Spectroscopy (ff-PWS) and Detection of Cancer Stages From Human Tissue Samples

TL;DR

This paper introduces a refined partial wave spectroscopy (PWS) technique that detects nanoscale structural changes in tissue samples, enabling early and accurate cancer stage diagnosis using commercially available tissue microarrays.

Contribution

The study develops a finer-focused PWS imaging method to quantify nanoscale tissue alterations, improving early cancer detection and staging accuracy.

Findings

Disorder strength increases with cancer stage.

Finer-focused PWS effectively detects nanoscale changes.

Potential for standardized early diagnosis.

Abstract

The progression of cancer is associated with different genetic and epigenetic events which result in nano to microscale structural alterations in cells/tissue. However, these structural alterations in the early stage of the disease remain undetectable by conventional microscopy due to the diffraction-limited resolution of ~200nm. With cancer being an epidemic worldwide, early and accurate detection methods are always in demand. In this sense, we developed a finer focusing mesoscopic physics-based partial wave spectroscopy (PWS) imaging and quantification technique, which can probe the precise scattering volume in cells/tissue to detect such structural alterations. Therefore, we employ the highly sensitive PWS technique to quantify the nanoscale refractive index fluctuations using commercially available paraffin embedded tissue microarrays (TMA) samples with the goal of the standardized early and accurate detection of stages of different cancers. Recently the use of scientific TMA samples has gained research interest in diagnosing different diseases and drug-effect in treatment since they are commercially easily accessible. In this work, deadly cancer such as pancreatic, prostate, breast, and colon cancer TMAs containing multiple cores of different stages for each cancer are analyzed using the PWS technique and the degree of disorder strength Ld was quantified. The finer focusing PWS results show that an increase in the disorder strength with the increase in the stage of each cancer. This quantitative analysis of different cancer stages using commercially available TMA samples could enhance and standardize early, accurate cancer diagnosis modalities.