Integrating microbial electrochemical technologies with anaerobic digestion to accelerate propionate degradation

TL;DR

This study evaluates how integrating microbial electrochemical technologies with anaerobic digestion affects propionate breakdown, methane production, and microbial communities, highlighting inoculum type impacts and energy considerations.

Contribution

It demonstrates the influence of different inocula on the performance and stability of integrated AD-MET systems for propionate degradation.

Findings

Anaerobic sludge inoculum maintained electrogenic activity and improved methane yield.

Pre-enriched electroactive consortium failed to sustain bioelectrochemical activity long-term.

AD-MET integration was not energetically favorable despite operational benefits.

Abstract

The aim of this study is to evaluate the integration of microbial electrochemical technologies (MET) with anaerobic digestion (AD) to overcome AD limitations caused by propionate accumulation. The study focuses on understanding to what extent the inoculum impacts on the behaviour of the integrated systems (AD-MET) from the perspective of propionate degradation, methane production and microbial population dynamics. Three different inocula were used: two from environmental sources (anaerobic sludge and river sediment) and another one from a pre-enriched electroactive consortium adapted to propionate degradation. Contrary to expectations, the reactor inoculated with the pre-enriched consortium was not able to maintain its initial good performance in the long run, and the bioelectrochemical activity collapsed after three months of operation. In contrast, the reactor inoculated with anaerobic sludge, although it required a relatively longer time to produce any observable current, was able to maintain the electrogenic activity operation (0.8 A.m-2) as well as the positive contribution of AD-MET integration to tackle propionate accumulation and to enhance methane yield (338 mL.gCOD-1). However, it must also be highlighted that from a purely energetic point of view the AD-MET was not favorable.