Multi-pulse Photoacoustic Microscopy Based on k-space Pseudospectral Method: Simulation Study

TL;DR

This study introduces multi-pulse photoacoustic microscopy using sequential laser pulses and k-space pseudospectral simulation, demonstrating improved SNR without increasing laser energy, thus enhancing biological imaging potential.

Contribution

It proposes a novel multi-pulse approach for photoacoustic microscopy and verifies its feasibility through simulation, expanding imaging capabilities.

Findings

SNR increases proportionally with the number of laser pulses

High SNR achieved with low-energy laser pulses

Multi-pulse method effective across A-scan, B-scan, and C-scan modes

Abstract

Photoacoustic imaging has emerged in the past decades. Compared with the traditional medical imaging mode, it has better imaging performance and has great development potential in the field of biological imaging. In traditional photoacoustic microscopy, a single laser pulse is generally used to irradiate the sample to produce photoacoustic signal. And signal-to-noise ratio (SNR) is a very important indicator for photoacoustic imaging. In order to obtain the image with high SNR, multiple acquisition or increasing laser pulse energy is usually adopted. The former will lead to slower imaging speed, and the latter will lead to photobleaching or phototoxicity. Here, we proposes multi-pulse photoacoustic microscopy, the photoacoustic signals were stimulated sequentially using multiple laser pulses in each A line data acquisition. In order to verify the feasibility of this method, a multi-pulse photoacoustic imaging simulation platform is established using k-Wave toolbox. The performance of multi-pulse photoacoustic imaging is verified through the three scanning modes of photoacoustic microscopy A-scan, B -scan, and C-scan. The results indicate that the SNR is proportion to the number of laser pulses used, high SNR can be achieved by low-energy laser pulse. This work will help to expand the application of photoacoustic imaging.