Inflammation-driven glial alterations in the cuprizone mouse model probed with diffusion-weighted magnetic resonance spectroscopy at 11.7 T

TL;DR

This study demonstrates that diffusion-weighted magnetic resonance spectroscopy (DW-MRS) can noninvasively detect inflammation-related glial alterations in a mouse model, with specific markers correlating to histological inflammation indicators.

Contribution

The paper shows that DW-MRS measurements of myo-inositol and choline diffusion are effective markers for glial cell changes in inflammation, providing a new noninvasive imaging approach.

Findings

mIns and tCho diffusion coefficients increased in cuprizone mice

Strong correlation between mIns diffusion and astrocytic changes

Moderate correlation between tCho diffusion and microglial alterations

Abstract

Inflammation of brain tissue is a complex response of the immune system to the presence of toxic compounds or to cell injury, leading to a cascade of pathological processes that include glial cell activation. Noninvasive magnetic resonance imaging markers of glial reactivity would be very useful for in vivo detection and monitoring of inflammation processes in the brain, as well as for evaluating the efficacy of personalized treatments. Due to their specific location in glial cells, myo-inositol (mIns) and choline compounds (tCho) seem the best candidates for probing glial-specific intra-cellular compartments. However, their concentrations quantified using conventional proton magnetic resonance spectroscopy (MRS) are not specific for inflammation. In contrast, it has been recently suggested that mIns intra-cellular diffusion, measured using diffusion-weighted MRS (DW-MRS) in a mouse model of reactive astrocytes, could be a specific marker of astrocytic hypertrophy. In order to evaluate the specificity of both mIns and tCho diffusion to inflammation-driven glial alterations, we performed DW-MRS in the corpus callosum of cuprizone-fed mice after 6 weeks of intoxication and evaluated the extent of astrocytic and microglial alterations using immunohistochemistry. Both mIns and tCho apparent diffusion coefficients were significantly elevated in cuprizone-fed mice compared to control mice, while histologic evaluation confirmed the presence of severe inflammation. Additionally, mIns and tCho diffusion showed, respectively, strong and moderate correlations with histological measures of astrocytic and microglial area fractions, confirming DW-MRS as a promising tool for specific detection of glial changes under pathological conditions.