Intracellular Electrochemical Nanomeasurements Reveal that Exocytosis of Molecules at Living Neurons is Subquantal and Complex

TL;DR

This study demonstrates that exocytosis in living neurons involves partial, subquantal release of molecules, revealing a complex and regulated process that contrasts with the traditional all-or-none model, with implications for neural communication.

Contribution

The paper provides direct electrochemical evidence that exocytosis in living neurons involves subquantal, partial release of neurotransmitters, challenging the classical all-or-none paradigm.

Findings

Only 4.5% of vesicular content is released in simple events.

10.7% of vesicular content is released in complex events.

Partial release allows for presynaptic plasticity and regulation.

Abstract

Since the early work of Bernard Katz, the process of cellular chemical communication via exocytosis, quantal release, has been considered to be all or none. Recent evidence has shown exocytosis to be partial or 'subquantal' at single-cell model systems, but there is a need to understand this at communicating nerve cells. Partial release allows nerve cells to control the signal at the site of release during individual events, where the smaller the fraction released, the greater the range of regulation. Here we show that the fraction of the vesicular octopamine content released from a living Drosophila larval neuromuscular neuron is very small. The percentage of released molecules was found to be only 4.5% for simple events and 10.7% for complex (i.e., oscillating or flickering) events. This large content, combined with partial release controlled by fluctuations of the fusion pore, offers presynaptic plasticity that can be widely regulated. Two works published in 2010 suggested that the Katz principle, [1] was incorrect for all-or-none release and that only part of the chemical load of vesicles was released during exocytosis, at least as measured as a full spike during amperometry. [2] The combination of electrochemical methods to measure both release and vesicle content in 2015 added a wealth of information to support the concept of partial release in exocytosis. [3] Additionally, this has recently been supported by work with TIRF microscopy showing 'subquantal' release from vesicles in adrenal chromaffin cells and using super-resolution STED microscopy. [4] It appears that the full event generally involves release of only part of the load of chemical messenger in single-cell model systems like adrenal chromaffin and PC12 cells. Is this also true at living neurons in a nervous system and to what extent? To answer this critical question, we quantified the number of octopamine molecules in the neuromuscular neurons of Drosophila larvae by adapting an amperometric technique developed in our