Effective behavior of cooperative and nonidentical molecular motors

TL;DR

This paper derives explicit analytical formulas for the effective transport properties of a cargo moved by two cooperative, nonidentical molecular motors, accounting for stochastic attachment-detachment dynamics and spatial configuration effects.

Contribution

It introduces a novel analytical framework using stochastic averaging and renewal theory to model cooperative, nonidentical molecular motors with detailed position jumps during attachment and detachment.

Findings

Analytical expressions match stochastic simulations well.

Formulas account for jumps during motor attachment/detachment.

Model applies to kinesin-1 and kinesin-2 under various loads.

Abstract

Analytical formulas for effective drift, diffusivity, run times, and run lengths are derived for an intracellular transport system consisting of a cargo attached to two cooperative but not identical molecular motors (for example, kinesin-1 and kinesin-2) which can each attach and detach from a microtubule. The dynamics of the motor and cargo in each phase are governed by stochastic differential equations, and the switching rates depend on the spatial configuration of the motor and cargo. This system is analyzed in a limit where the detached motors have faster dynamics than the cargo, which in turn has faster dynamics than the attached motors. The attachment and detachment rates are also taken to be slow relative to the spatial dynamics. Through an application of iterated stochastic averaging to this system, and the use of renewal-reward theory to stitch together the progress within each switching phase, we obtain explicit analytical expressions for the effective drift, diffusivity, and processivity of the motor-cargo system. Our approach accounts in particular for jumps in motor-cargo position that occur during attachment and detachment events, as the cargo tracking variable makes a rapid adjustment due to the averaged fast scales. The asymptotic formulas are in generally good agreement with direct stochastic simulations of the detailed model based on experimental parameters for various pairings of kinesin-1 and kinesin-2 under assisting, hindering, or no load.