Optimal surface topography for cell adhesion is driven by cell membrane mechanics

TL;DR

This study presents a model linking cell membrane mechanics to optimal titanium nanotube surface topographies, predicting diameters that enhance cell adhesion and proliferation by minimizing membrane deformation energy.

Contribution

The paper introduces a new model incorporating cell membrane mechanics and a dimensionless parameter to predict optimal nanotube diameters for cell adhesion.

Findings

Optimal nanotube diameters range from 20 nm to 100 nm.

The cell interaction index correlates with membrane deformation energy.

Surface topologies can be tuned to selectively promote certain cell types.

Abstract

Titanium surface treated with titanium oxide nanotubes was used in many studies to quantify the effect of surface topography on cell fate. However, the predicted optimal diameter of nanotubes considerably differs among studies. We propose a model that explain cell adhesion to nanostructured surface by considering deformation energy of cell protrusions into titanium nanotubes and adhesion to surface. The optimal surface topology is defined as a geometry that gives membrane a minimum energy shape. A dimensionless parameter, the cell interaction index, was proposed to describe interplay between the cell membrane bending, intrinsic curvature and strength of cell adhesion. Model simulation show that optimal nanotube diameter ranging from 20 nm to 100 nm (cell interaction index between 0.2 and 1, respectively) is feasible within certain range of parameters describing adhesion and bending energy. The results indicates a possibility to tune the topology of nanostructural surface in order to enhance proliferation and differentation of cells mechanically compatible with given surface geometry while suppress the growth of other mechanically incompatible cells.