Wide-field fluorescent nanodiamond spin measurements toward real-time large-area intracellular quantum thermometry

TL;DR

This paper demonstrates that wide-field ODMR detection with proper ROI management can achieve real-time, large-area intracellular quantum thermometry in living cells, comparable to confocal methods.

Contribution

It shows how optimizing camera ROI and parameters enables wide-field ODMR to perform accurate, real-time thermometry over large cellular areas.

Findings

ROI shape and size significantly affect thermometry accuracy

Proper ROI and parameter management achieve confocal-level precision

Wide-field ODMR enables real-time large-area cellular thermometry

Abstract

In this study, we analyze the operational process of nanodiamond (ND) quantum thermometry based on wide-field detection of optically detected magnetic resonance (ODMR) of nitrogen vacancy centers, and compare its performance with that of confocal ODMR detection. We found that (1) the thermometry results are significantly affected by the shape and size of the camera region of interest (ROI) surrounding the target NDs and that (2) by properly managing the ROI and acquisition parameters of the camera, a temperature precision comparable to confocal detection in living cells can be obtained by wide-field ODMR. Our results are significant to the development of camera-based real-time large-area quantum thermometry of living cells.