Inline holographic microscopy through fiber imaging bundles

TL;DR

This paper presents a simple, calibration-free method for inline holographic microscopy through fiber bundles using partially coherent light, enabling volumetric imaging with high resolution and bending insensitivity.

Contribution

It introduces a novel, straightforward approach for holographic imaging through fiber bundles without complex calibration, suitable for miniaturized endoscopic applications.

Findings

Achieves ~6 um resolution over 1 mm depth range

Does not require complex calibration or phase conjugation

Insensitive to fiber bending effects

Abstract

Fiber imaging bundles are widely used as thin, passive image conduits for miniaturised and endoscopic microscopy, particularly for confocal fluorescence imaging. Holographic microscopy through fiber bundles is more challenging; phase conjugation approaches are complex and require extensive calibration. This articles describes how simple inline holographic microscopy can be performed through an imaging bundle using a partially coherent illumination source from a multimode fiber. The sample is imaged in transmission, with the intensity hologram sampled by the bundle and transmitted to a remote camera. The hologram can then be numerically refocused for volumetric imaging, achieving a resolution of approximately 6 um over a depth range of 1 mm. The scheme does not require any complex prior calibration and hence is insensitive to bending.