A minimal model for structure, dynamics, and tension of monolayered cell colonies

TL;DR

This paper introduces a minimal active Brownian particle model with attraction to simulate monolayered cell colonies, capturing their fluid-like interior, cohesive edge behavior, and collective dynamics observed experimentally.

Contribution

It presents a novel minimal active particle model that reproduces key features of cell colonies, including fluid-vacuum coexistence and collective motion, with insights into tension and detachment phenomena.

Findings

Model generates fluid-vacuum coexistence at moderate propulsion.

Colony exhibits outward cell polarity and tensile stress.

Strong propulsion leads to cell cluster detachment.

Abstract

The motion of cells in tissues is an ubiquitous phenomenon. In particular, in monolayered cell colonies in vitro, pronounced collective behavior with swirl-like motion has been observed deep within a cell colony, while at the same time, the colony remains cohesive, with not a single cell escaping at the edge. Thus, the colony displays liquid-like properties inside, in coexistence with a cell-free "vacuum" outside. How can adhesion be strong enough to keep cells together, while at the same time not jam the system in a glassy state? What kind of minimal model can describe such a behavior? Which other signatures of activity arise from the internal fluidity? We propose a novel active Brownian particle model with attraction, in which the interaction potential has a broad minimum to give particles enough wiggling space to be collectively in the fluid state. We demonstrate that for moderate propulsion, this model can generate the fluid-vacuum coexistence described above. In addition, the combination of the fluid nature of the colony with cohesion leads to preferred orientation of the cell polarity, pointing outward, at the edge, which in turn gives rise to a tensile stress in the colony -- as observed experimentally for epithelial sheets. For stronger propulsion, collective detachment of cell clusters is predicted. Further addition of an alignment preference of cell polarity and velocity direction results in enhanced coordinated, swirl-like motion, increased tensile stress and cell-cluster detachment.