Blocking of the CD80/86 axis as a therapeutic approach to prevent progression to more severe forms of COVID-19

TL;DR

This study suggests that blocking the CD80/86 immune checkpoint could be a promising therapeutic approach to prevent severe COVID-19 by reducing cytokine storms and inflammatory responses, based on integrated transcriptomic analyses.

Contribution

It provides in-silico evidence that inhibiting CD80/86 co-stimulation may mitigate severe COVID-19 inflammation, supporting potential therapeutic strategies.

Findings

CD80/86 inhibition reverses COVID-19 associated biological processes.

Abatacept response antagonizes COVID-19 transcriptional signatures.

Correlation between CD80/86 axis components and IL6 production in COVID-19 cells.

Abstract

In its more severe forms, COVID-19 progresses towards an excessive immune response, leading to the systemic overexpression of proinflammatory cytokines like IL6, mostly from the infected lungs. This cytokine storm can cause multiple organ damage and death. Consequently, there is a pressing need to identify therapies to treat and prevent severe symptoms during COVID-19. Based on previous clinical evidence, we hypothesized that inhibiting T cell co-stimulation by blocking CD80/86 could be an effective therapeutic strategy against progression to severe proinflammatory states. To support this hypothesis, we performed an analysis integrating blood transcriptional data we generated from rheumatoid arthritis patients treated with abatacept -- a CD80/86 co-stimulation inhibitor -- with the pathological features associated with COVID-19, particularly in its more severe forms. We have found that many of the biological processes that have been consistently associated with COVID-19 pathology are reversed by CD80/86 co-stimulation inhibition, including the downregulation of IL6 production. Also, analysis of previous transcriptional data from blood of SARS-CoV-infected patients showed that the response to abatacept has a very high level of antagonism to that elicited by COVID-19. Finally, analyzing a recent single cell RNA-seq dataset from bronchoalveolar lavage fluid cells from COVID-19 patients, we found a significant correlation along the main elements of the C80/86 axis: CD86+/80+ antigen presenting cells, activated CD4+ T cells and IL6 production. Our in-silico study provides additional support to the hypothesis that blocking of the CD80/CD86 signaling axis may be protective of the excessive proinflammatory state associated with COVID-19 in the lungs.