Free Radical Scavenging and Cytotoxic Activities of Substituted Pyrimidines

TL;DR

This study synthesized substituted pyrimidines that exhibit strong free radical scavenging and cytotoxic activities, suggesting potential as cancer-inhibitory compounds targeting reactive oxygen species.

Contribution

It reports the synthesis and evaluation of novel substituted pyrimidines with both antioxidant and cytotoxic properties, establishing structure-activity relationships.

Findings

All compounds showed good free radical scavenging activity.

Most analogues exhibited cytotoxicity in 3T3 cells.

Identified novel pyrimidines with potential anticancer activity.

Abstract

A library of substituted pyrimidines was synthesized and evaluated for free radical scavenging, and in vitro cytotoxic activity in 3T3 cells. All compounds showed good free radical scavenging activity with IC50 values in the range of 42.9 + 0.31 to 438.3 3.3 {\mu}M as compared to the standard butylated hydroxytoluene having IC50 value of 128.83 2. 1 {\mu}M. The structure activity-relationship was also established. Selected analogues 1, 2, 3, 5, 6, 7, 8, 9, 10, 12, 13, 15, 19, 20, 21, 24, 25, 26 and 28 were tested for cytotoxicity in mouse fibroblast 3T3 cell line using MTT assay, and most of the analogues showed cytotoxicity. This study has identified a number of cytotoxic novel substituted pyrimidines having free radical scavenging activities that can be used as inhibitory compounds for those cancer cells whose growth is mediated by reactive oxygen species.