Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids
Jules Scholler, Kassandra Groux, Olivier Goureau, Jos\'e-Alain Sahel,, Mathias Fink, Sacha Reichman, Claude Boccara, Kate Grieve
TL;DR
This paper introduces dynamic full-field optical coherence tomography for non-invasive, high-resolution 3D live imaging of retinal organoids, providing functional insights through organelle motility visualization.
Contribution
It presents a novel imaging technique that combines high spatial and temporal resolution with functional contrast to study living retinal tissue.
Findings
Colored images reveal organelle motility linked to cell function
Sub-micrometer spatial resolution achieved
Millisecond temporal resolution enables real-time imaging
Abstract
Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue, but lacks functional information. We present dynamic full-field optical coherence tomography to image living human induced pluripotent stem cell-derived retinal organoids non-invasively. Colored images with an endogenous contrast linked to organelle motility are generated, with sub-micrometer spatial resolution and millisecond temporal resolution, opening an avenue to identify specific cell types in living tissue via their function.
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