Squeezed Light Induced Two-photon Absorption Fluorescence of Fluorescein Biomarkers

TL;DR

This paper demonstrates that using high-flux squeezed light sources significantly enhances two-photon absorption fluorescence in fluorescein biomarkers, surpassing classical methods and revealing new behaviors in TPA rates.

Contribution

The study introduces the use of high-flux squeezed light for TPA, achieving substantial fluorescence enhancement and revealing polynomial TPA rate behavior.

Findings

Achieved 47-fold fluorescence enhancement with squeezed light.

Observed polynomial TPA rate behavior in DCM laser dye.

Demonstrated advantages over entangled TPA with SPDC sources.

Abstract

Two-photon absorption (TPA) fluorescence of biomarkers has been decisive in advancing the fields of biosensing and deep-tissue in vivo imaging of live specimens. However, due to the extremely small TPA cross section and the quadratic dependence on the input photon flux, extremely high peak-intensity pulsed lasers are imperative, which can result in significant photo- and thermal-damage. Previous works on entangled TPA (ETPA) with spontaneous parametric down-conversion (SPDC) light sources found a linear dependence on the input photon-pair flux, but are limited by low optical powers, along with a very broad spectrum. We report that by using a high-flux squeezed light source for TPA, a fluorescence enhancement of 47 is achieved in fluorescein biomarkers as compared to classical TPA. Moreover, a polynomial behavior of the TPA rate is observed in the DCM laser dye.