Using the Sub-Glass Transition Vibrational Dynamics to Predict Protein Stability in the Solid State: Fact or Fiction?

TL;DR

This study investigates whether sub-glass transition vibrational dynamics, specifically $eta$-relaxation, can predict protein stability in solid formulations by linking molecular motions to stability data over a year.

Contribution

It provides a novel connection between the potential energy surface shape, vibrational dynamics, and long-term protein stability in solid state formulations.

Findings

$eta$-relaxation motions are linked to protein stability.

Terahertz spectroscopy and DMA reveal dynamics correlated with stability.

The study offers insights into predicting stability based on vibrational properties.

Abstract

The $\beta$-relaxation associated with the sub-glass transition temperature ($T_{\text{g},\beta}$) is attributed to fast, localised molecular motions which can occur below the primary glass transition temperature ($T_{g\alpha}$). Despite $T_{\text{g},\beta}$ being observed well-below storage temperatures, the $\beta$-relaxation associated motions have been hypothesised to influence protein stability in the solid state and could thus impact the quality of, e.g. protein powders for inhalation or reconstitution and injection. However, to date, there is no comprehensive explanation in the literature which answers the question: How is protein stability during storage influenced by the $\beta$-relaxation? Here, we connect the shape of the potential energy surface (PES) to data obtained from our own (storage) stability study, to answer this question. The 52-week stability study was conducted on a selection of multi-component monoclonal antibody (mAb) formulations. Solid state dynamics of the formulations were probed using terahertz time-domain spectroscopy (THz-TDS) and dynamic mechanical analysis (DMA).