Guidelines for reporting single-cell RNA-Seq experiments

TL;DR

This paper proposes standardized guidelines for reporting single-cell RNA-Seq experiments, focusing on essential metadata to enhance reproducibility of data analysis across studies.

Contribution

It introduces a minimum set of metadata requirements for scRNA-Seq experiments to improve reproducibility and data sharing.

Findings

Defined essential metadata for scRNA-Seq reporting

Facilitates reproducibility of single-cell studies

Supports data sharing in public archives

Abstract

Single-cell RNA-Sequencing (scRNA-Seq) has undergone major technological advances in recent years, enabling the conception of various organism-level cell atlassing projects. With increasing numbers of datasets being deposited in public archives, there is a need to address the challenges of enabling the reproducibility of such data sets. Here, we describe guidelines for a minimum set of metadata to sufficiently describe scRNA-Seq experiments, ensuring reproducibility of data analyses.