Models for membrane curvature sensing of curvature generating proteins

TL;DR

This paper compares two models of membrane curvature sensing by proteins, analyzing their predictions and applicability to different biological mechanisms through analytical and simulation methods.

Contribution

It provides a detailed comparison of the spontaneous curvature and curvature mismatch models, clarifying their assumptions, predictions, and suitable biological contexts.

Findings

Spontaneous curvature model predicts a monotonic sensing curve.

Curvature mismatch model predicts a non-monotonic sensing curve.

Different models are suited for different protein mechanisms.

Abstract

The curvature sensitive localization of proteins on membranes is vital for many cell biological processes. Coarse-grained models are routinely employed to study the curvature sensing phenomena and membrane morphology at the length scale of few micrometers. Two prevalent phenomenological models exist for modeling experimental observations of curvature sensing, (1) the spontaneous curvature model and (2) the curvature mismatch model, which differ in their treatment of the change in elastic energy due to the binding of proteins on the membrane. In this work, the prediction of sensing and generation behaviour, by these two models, are investigated using analytical calculations as well as Dynamic Triangulation Monte Carlo simulations of quasi-spherical vesicles. While the spontaneous curvature model yields a monotonically decreasing sensing curve as a function of vesicle radius, the curvature mismatch model results in a non-monotonic sensing curve. We highlight the main differences in the interpretation of the protein-related parameters in the two models. We further propose that the spontaneous curvature model is appropriate for modeling peripheral proteins employing the hydrophobic insertion mechanism, with minimal modification of membrane rigidity, while the curvature mismatch model is appropriate for modeling curvature generation using scaffolding mechanism where there is significant stiffening of the membrane due to protein binding.