Does inter-organellar proteostasis impact yeast quality and performance during beer fermentation?

TL;DR

This paper explores how inter-organellar proteostasis mechanisms in yeast influence yeast health and performance during high-gravity beer fermentation, highlighting the interconnected stress responses in ER, mitochondria, and cytosol.

Contribution

It provides a comprehensive hypothesis linking inter-organellar proteostasis to yeast viability and performance in beer fermentation, supported by transcriptome data analysis.

Findings

Upregulation of chaperones and stress response genes during fermentation

Activation of proteostasis pathways in ER, mitochondria, and cytosol

Potential impact on yeast vitality and fermentation efficiency

Abstract

During beer production, yeast generate ethanol that is exported to the extracellular environment where it accumulates. Depending on the initial carbohydrate concentration in the wort a high concentration of ethanol can be achieved in beer, often higher than 20% (v/v). It is noteworthy that the effects of elevated ethanol concentrations generated during beer fermentation resemble those of heat shock stress, with similar responses observed in both situations, such as the activation of proteostasis and protein quality control mechanisms in different cell compartments, including endoplasmic reticulum (ER), mitochondria, and cytosol. Despite the extensive published molecular and biochemical data regarding the roles of proteostasis in different organelles of yeast cells, little is known about how this mechanism impacts beer fermentation and how different proteostasis mechanisms found in ER, mitochondria, and cytosol communicate with each other during ethanol/fermentative stress. Supporting this integrative view, transcriptome data analysis was applied using publicly available information for a lager yeast strain grown under in beer production conditions. The transcriptome data indicated upregulation of genes that encode chaperones, co-chaperones, unfolded protein response elements in ER and mitochondria, ubiquitin ligases, proteasome components, N-glycosylation quality control pathway proteins, and components of processing bodies (p-bodies) and stress granules (SGs) during lager beer fermentation. Thus, the main purpose of this hypothesis and theory manuscript is to provide a concise picture of how inter-organellar proteostasis mechanisms are connected with one another and with biological processes that may modulate the viability and/or vitality of yeast populations during HG/VHG beer fermentation and serial repitching.