Noncovalent force spectroscopy using wide-field optical and diamond-based magnetic imaging

TL;DR

This paper introduces a novel force spectroscopy method combining wide-field optical and diamond-based magnetic imaging with NV centers to detect biomolecular interactions, enabling high-throughput nanoscale sample screening.

Contribution

It presents a new FIRMS technique utilizing NV center magnetometry for parallel, sensitive detection of biomolecular forces.

Findings

Demonstrates detection of biomolecular binding via force-induced magnetization.

Shows potential for scalable, high-throughput nanoscale screening.

Integrates optical and magnetic imaging for enhanced sensitivity.

Abstract

A realization of the force-induced remnant magnetization spectroscopy (FIRMS) technique of specific biomolecular binding is presented where detection is accomplished with wide-field optical and diamond-based magnetometry using an ensemble of nitrogen-vacancy (NV) color centers. The technique may be adapted for massively parallel screening of arrays of nanoscale samples.