# On the influence of device handle in single-molecule experiments

## TL;DR

This paper presents an analytical model to understand how the stiffness of device handles affects measurements in single-molecule force spectroscopy, highlighting potential errors in stability and transition threshold estimations.

## Contribution

The study introduces a fully analytical model that predicts measurement artifacts caused by handle stiffness in single-molecule experiments.

## Key findings

- Neglecting handle stiffness leads to significant measurement errors.
- Handle stiffness impacts the estimation of molecular stability.
- The model helps correct for artifacts in experimental data.

## Abstract

We deduce a fully analytical model to predict the artifacts of the measuring device handles in Single Molecule Force Spectroscopy experiments. As we show, neglecting the effects of the handle stiffness can lead to crucial overestimation or underestimation of the stability properties and transition thresholds of macromolecules.