Purification of total flavonoids from Aurea Helianthus flowers and In Vitro Hypolipidemic Effect

TL;DR

This study optimized purification of flavonoids from Aurea Helianthus flowers and demonstrated their strong bile acid binding ability, indicating potential as natural hypolipidemic agents.

Contribution

It introduces an effective purification method for flavonoids and links their purity to hypolipidemic potential through bile acid binding analysis.

Findings

Purity of flavonoids increased from 27.8% to 83.5% after purification.

Purified flavonoids showed high binding rates to cholates, up to 88.2%.

Flavonoid purity correlates strongly with bile acid binding capacity.

Abstract

The effects of purification methods and its hypolipidemic function on the total flavonoids of Aurea Helianthus flower were investigated. Liquid-liquid extraction of ethanol extract from Aurea Helianthus flower was carried out by using different polar solvents. The extract with the highest total flavonoid content was selected, and the optimal conditions for purification of total flavonoids were determined by purification with macroporous resin. The human digestive environment was simulated in vitro, and the binding ability of different flavonoid samples to three kinds of cholate was compared. The results showed that the purity of total flavonoids in ethanol extract was 27.8%, the purity of total flavonoids in ethyl acetate extract was 46.4%, and the purity was increased by 18.6%. Subsequent purification with AB-8 macroporous resin; loading of total flavonoids at a concentration of 5.5 mg/mL, flow rate of 1.5 mL/min, 110 mL; use of 75% ethanol, 80 mL as eluent at a flow rate of 1.5 mL The elution at /min resulted in a total flavonoid purity of 83.5 % and an increase of 37.1%, and a good purification effect was obtained. The binding rate of total flavonoids purified by AB-8 macroporous resin to sodium taurocholate, sodium glycocholate and sodium cholate was 88.2%, 73.2% and 75.8 %, respectively. The binding ability was the strongest, and the others were ethyl acetate. Extract, ethanol extract. The purity of total flavonoids showed a good correlation with the binding capacity of cholate, and the correlation coefficient was between 0.963 and 0.988. The total flavonoids of Aurea Helianthus flower have good bile acid binding ability and can be used as the focus of natural hypolipidemic substances.