Effect of different polarity solvents on total phenols and flavonoids content, and In-vitro antioxidant properties of flowers extract from Aurea Helianthus

TL;DR

This study investigates how different polar solvents affect the extraction of phenols and flavonoids from Aurea Helianthus flowers and evaluates their antioxidant properties, highlighting ethyl acetate as the most effective solvent.

Contribution

It provides new insights into the optimal polar solvents for extracting phenols and flavonoids with antioxidant activity from Aurea Helianthus flowers.

Findings

Ethyl acetate extract had the highest phenols and flavonoids content.

All extracts showed significant antioxidant activity, especially ethyl acetate and chloroform.

Good correlation between phenols, flavonoids, and antioxidant activity.

Abstract

The total phenols and flavonoids content of different polar solvent extracts from Aurea Helianthus flowers, and their antioxidant activity were determined. The ethanol extract of Aurea Helianthus flowers were suspended in water and fractionated using different polar solvents; hexane, chloroform, ethyl acetate, butanol and water. The parameters of each extract mentioned above were determined using Floin-ciocalteu reagent(FCR) method, AlCl3 colorimetry method, ferric reducing ability of plasma(FRAP) assay, total antioxidant activity(TAA) assay and DPPH radical scavenging assay. The highest total phenols content(516.21 mg GAE/g) and flavonoids content(326.06 mg QCE/g) were obtained in ethyl acetate extract, the correlation between TPC and TFC assay was founded to be 0.967. All polar solvent extracts of Aurea Helianthus flowers showed significant antioxidant effects, the hightest inhibition was obtained in ethyl acetate and choroform extracts and the lowest inhibition in the water extract. There is a good correlation of total phenols and flavonoids content with antioxidant activity. This work indicated that the polar solvent extracts of Aurea Helianthus flowers contain high phenols and flavonoids content and exhibited antioxidant activities in vitro, therefore, could be candidates for use as natural antioxidant.