Optical synthetic sampling imaging: concept and an example of microscopy

TL;DR

This paper introduces a synthetic sampling imaging (SSI) method that combines structured illumination and detection to overcome undersampling in digital microscopy, effectively doubling the resolution of the images.

Contribution

The paper presents a novel SSI concept that synthesizes illumination and detection sampling rates to enhance image resolution in microscopy.

Findings

Successfully doubled the sampling resolution of a microscope.

Demonstrated the effectiveness of SSI in resolving undersampling issues.

Provides a new approach for high-resolution digital imaging.

Abstract

Digital two-dimensional (2D) spatial sampling devices (such as charge-coupled device) have been widely used in various imaging systems, especially in computational imaging systems. However, the undersampling of digital sampling devices is a problem that limits the resolution of the acquired images. In this study, we present a synthetic sampling imaging (SSI) concept to solve the undersampling problem. It combines the structured illumination system and conventional 2D image detection system to simultaneously sample the specimen from the illumination and the detection sides. Then, we synthesize the illumination sampling rate and the detection sampling rate to reconstruct a high sampling rate image. The concept of the proposed SSI is demonstrated by an example of microscopy. Experimental results confirm that the proposed method can double the sampling resolution of the microscope. The synthetic sampling scheme, where the sampling task is shared by the illumination and detection sides, provides insight for resolving the undersampling problem of the digital imaging system.