On-Chip Concentration and Patterning of Biological Cells Using Interplay of Electrical and Thermal Fields

TL;DR

This paper presents a novel on-chip method for concentrating and patterning biological cells using combined electrical and thermal fields, avoiding external heating or illumination, and enabling rapid, safe cell manipulation for biomedical applications.

Contribution

The study introduces a new technique that exploits electrothermal effects with simple electrode design to manipulate cells without external heating or light sources.

Findings

Effective concentration of E. coli and yeast cells demonstrated.

Cells patterned into various shapes based on design parameters.

Method operates within safe temperature limits for biological samples.

Abstract

We demonstrate a method of concentrating and patterning of biological cells on a chip, exploiting the confluence of electric and thermal fields, without necessitating the use of any external heating or illuminating source. The technique simply employs two parallel plate electrodes and an insulating layer over the bottom electrode, with a drilled insulating layer for inducing localized variations in the thermal field. A strong induced electric field, in the process, penetrates through the narrow hole and generates highly non-uniform heating, which in turn, results in gradients in electrical properties and induces mobile charges to impose directional fluid flow. The toroidal vortices, induced by secondary electrokinetic forces originating out of temperature-dependent electrical property variations, transport the suspended cells towards a hot-spot site of the chip, for rapid concentrating and patterning into different shaped clusters based on pre-designed conditions, without exceeding safe temperature limits that do not result in damage of thermally labile biological samples. We characterize the efficacy of the cell trapping process for two different biological entities, namely, Escherichia coli bacteria and yeast cell. These results may be of profound importance towards developing novel biomedical microdevices for drug discovery, antibiotic resistance assessment and medical diagnostics.