Paper-based cell cryopreservation

TL;DR

This paper introduces a simple, cost-effective paper-based method for cell cryopreservation that enhances cell recovery and supports 3D culture preservation, offering a practical alternative to traditional techniques.

Contribution

It presents a novel paper-based cryopreservation platform that is easy to use, cost-effective, and compatible with 3D cell cultures, requiring no complex modifications.

Findings

Fibronectin treatment improves cell release after thawing.

Cells within paper form spheroid-like structures post-thaw.

Method effectively preserves pre-formed 3D cultures.

Abstract

The continuous development of simple and practical cell cryopreservation methods is of great importance to a variety of sectors, especially when considering the efficient short- and long-term storage of cells and their transportation. Although the overall success of such methods has been increased in recent years, there is still need for a unified platform that is highly suitable for efficient cryogenic storage of cells in addition to their easy-to-manage retrieval. Here, we present a paper-based cell cryopreservation method as an alternative to conventional cryopreservation methods. The method is space saving, cost-effective, simple and easy to manage, and requires no additional fine-tuning to conventional freezing and thawing procedures to yield comparable recovery of viable cells. We show that treating papers with fibronectin solution induces enhanced release of viable cells post thawing as compared to untreated paper platforms. Additionally, upon release, the remaining cells within paper lead to the formation and growth of spheroid-like structures. Moreover, we demonstrate that the developed method works with paper-based 3D cultures, where pre-formed 3D cultures can be efficiently cryopreserved.