Long-term stable poly(ionic liquid)/MWCNTs inks enable enhanced surface modification for electrooxidative detection and quantification of dsDNA

TL;DR

This study develops stable poly(ionic liquid)/MWCNT inks for surface modification of electrodes, enabling sensitive electrochemical detection of dsDNA and single point mutations, with potential clinical applications.

Contribution

Introduces long-term stable PIL/MWCNT inks for electrode modification, enhancing electrochemical DNA detection and mutation sensing capabilities.

Findings

Enhanced surface area and electron transfer on modified electrodes.

Linear detection range for dsDNA is 5-500 μg/mL for guanine.

Capable of detecting single point mutations in DNA fragments.

Abstract

This work demonstrates the use of imidazolium-based poly(ionic liquid)s (PILs) as efficient dispersants of multi-walled carbon nanotubes (MWCNTs). With these polymeric dispersants, highly stable fine dispersions of MWCNTs (inks) can be easily prepared in aqueous media and applied for rather simple but efficient surface modification of screen-printed electrodes (SPEs). Such a modification of SPEs remarkably increases the electroactive surface area and accelerates the electron transfer rate due to synergistic combination of specific features of MWCNTs such as strong adsorptive property and high specific surface with the advantages of PILs like ion conductivity and dispersability. We further show that the PIL/MWCNT-modified SPEs can be beneficially utilized for direct electrochemical analysis of double stranded DNA (dsDNA). Specifically, it is exemplified by the direct electrooxidation of guanine and adenine bases in salmon testes dsDNA chosen as a model system. The linear ranges for the determination of dsDNA correspond to 5-500 microg per mL for the oxidative peak of guanine and 0.5-50 microg per mL for the oxidative peak of adenine. This makes direct electrochemical dsDNA detection with the use of the easy-preparable PIL/MWCNT-modified SPEs strongly competing to currently applied spectral and fluorescent techniques. Furthermore, we show that the developed constructs are capable of sensing a single point mutation in the 12-bases single-stranded DNA fragments. Such detection is of high clinical significance in choosing an adequate anticancer treatment, where the electrochemical identification of the point mutation could offer time and cost benefits.