The emergence of sequence-dependent structural motifs in stretched, torsionally constrained DNA

TL;DR

This study uses molecular dynamics simulations to reveal how DNA sequence influences structural motifs and stability under mechanical stress, highlighting sequence-dependent responses during stretching and supercoiling relevant to cellular functions.

Contribution

It demonstrates that DNA sequence determines the formation of specific structural motifs and stability during mechanical perturbations, a novel insight into sequence-dependent DNA mechanics.

Findings

GC-rich sequences are more stable than AT-rich under stress.

Sequence influences formation of non-canonical hydrogen bonds and stacking.

DNA denatures at 20-30% extension, affecting cellular processes.

Abstract

The double-helical structure of DNA results from canonical base pairing and stacking interactions. However, variations from steady-state conformations result from mechanical perturbations in cells. These different topologies have physiological relevance but their dependence on sequence remains unclear. Here, we use molecular dynamics simulations to show that sequence differences result in markedly different structural motifs upon physiological twisting and stretching. We simulated overextension on four different sequences of DNA ((AA)12, (AT)12, (GG)12 and (GC)12) with supercoiling densities within the physiological range. We found that DNA denatures in the majority of stretching simulations, surprisingly including those with overtwisted DNA. GC-rich sequences were observed to be more stable than AT-rich, with the specific response dependent on base pair ordering. Furthermore, we found that (AT)12 forms stable periodic structures with non-canonical hydrogen bonds in some regions and non-canonical stacking in others, whereas (GC)12 forms a stacking motif of four base pairs independent of supercoiling density. Our results demonstrate that 20-30% DNA extension is sufficient for breaking B-DNA around and significantly above cellular supercoiling, and that the DNA sequence is crucial for understanding structural changes under mechanical stress. Our findings have important implications for the activities of protein machinery interacting with DNA in all cells.