Simultaneous off-axis multiplexed holography and regular fluorescence microscopy of biological cells

TL;DR

This paper introduces a novel multimodal microscopy technique that combines quantitative phase imaging and fluorescence microscopy simultaneously using a single camera, enhancing biological cell analysis.

Contribution

The method enables concurrent phase and fluorescence imaging with conventional fluorescence compatibility, using an external interferometric multiplexing module, unlike previous interferometric fluorescence approaches.

Findings

Successful imaging of fluorescent beads and live cells

Simultaneous acquisition of phase and fluorescence data

Compatibility with standard fluorescence microscopy

Abstract

We present a new technique for obtaining simultaneous multimodal quantitative phase and fluorescence microscopy of biological cells, providing both quantitative phase imaging and molecular specificity using a single camera. Our system is based on an interferometric multiplexing module, externally positioned at the exit of an optical microscope. In contrast to previous approaches, the presented technique allows conventional fluorescence imaging, rather than interferometric off-axis fluorescence imaging. We demonstrate the presented technique for imaging fluorescent beads and live biological cells.