# Modeling transport and mean age of dense core vesicles in large axonal arbors

## TL;DR

This paper presents a computational model of dense core vesicle transport in Drosophila motoneuron axonal terminals, revealing insights into vesicle distribution, age, and circulation, with implications for understanding neuronal transport mechanisms.

## Contribution

The model uniquely simulates DCV concentrations, fluxes, and age distributions in large axonal boutons, highlighting differences from experimental steady-state observations.

## Key findings

- DCV circulation predicted after terminal saturation, unlike experimental data.
- Most DCVs are in the resident state rather than transiting.
- Transiting DCVs may serve as a reserve for replenishing boutons.

## Abstract

A model simulating transport of dense core vesicles (DCVs) in type II axonal terminals of Drosophila motoneurons has been developed. The morphology of type II terminals is characterized by the large number of en passant boutons. The lack of both scaled up DCV transport and scaled down DCV capture in boutons results in a less efficient supply of DCVs to distal boutons. Furthermore, the large number of boutons that DCVs pass as they move anterogradely, until they reach the most distal bouton, may lead to the capture of a majority of DCVs before they turn around in the most distal bouton to move in the retrograde direction. This may lead to a reduced retrograde flux of DCVs and a lack of DCV circulation in type II terminals. The developed model simulates DCV concentrations in boutons, DCV fluxes between the boutons, age density distributions of DCVs, and the mean age of DCVs in various boutons. Unlike published experimental observations, our model predicts DCV circulation in type II terminals after these terminals are filled to saturation. This disagreement is likely because experimentally observed terminals were not at steady-state, but rather were accumulating DCVs for later release. Our estimates show that the number of DCVs in the transiting state is much smaller than that in the resident state. DCVs traveling in the axon, rather than DCVs transiting in the terminal, may provide a reserve of DCVs for replenishing boutons after a release. The techniques for modeling transport of DCVs developed in our paper can be used to model the transport of other organelles in axons.

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Source: https://tomesphere.com/paper/1903.10120