# Deep penetration fluorescence imaging through dense yeast cells suspensions using Airy beams

## TL;DR

This paper introduces a novel Airy beam scanning method for high-resolution fluorescence imaging through dense, turbid media, outperforming confocal imaging in clarity and depth measurement capabilities.

## Contribution

The study presents a new imaging technique utilizing Airy beams' non-diffractive properties to achieve clearer, deeper fluorescence imaging in scattering media, with depth measurement without additional sectioning.

## Key findings

- Successfully imaged 1μm fluorescent beads through dense yeast suspensions.
- Achieved higher resolution and signal-to-noise ratio than confocal imaging.
- Demonstrated depth measurement of imaged objects without extra sectioning.

## Abstract

We propose a new method to image fluorescent objects through turbid media base on Airy beam scanning. This is achieved by using the non-diffractive nature of Airy beams, namely their ability to maintain their shape while penetrating through a highly scattering medium. We show, that our technique can image fluorescent objects immersed in turbid media with higher resolution and signal to noise than confocal imaging. As proof-of-principle, we demonstrate imaging of 1$\mu$m sized fluorescent beads through a dense suspension of yeast cells with an attenuation coefficient of 51cm$^{-1}$ at a depth of 90$\mu$m. Finally, we demonstrate that our technique can also provide the depth of the imaged object without any additional sectioning.