Afferent Fiber Activity-Induced Cytoplasmic Calcium Signaling in Parvalbumin-Positive Inhibitory Interneurons of the Spinal Cord Dorsal Horn

TL;DR

This study develops methods to visualize calcium signaling in specific inhibitory neurons of the spinal cord dorsal horn, providing insights into their role in pain sensitization and potential targets for chronic pain treatment.

Contribution

The paper introduces viral vector-based techniques for measuring calcium signals in genetically defined spinal inhibitory neurons, advancing understanding of their role in pain mechanisms.

Findings

Ca2+ signals are triggered by afferent fiber activity in inhibitory neurons

Methods enable precise characterization of calcium dynamics in specific neuron types

Potential for future studies to manipulate calcium signaling in pain pathways

Abstract

Neuronal calcium (Ca2+) signaling represents a molecular trigger for diverse central nervous system adaptations and maladaptions. The altered function of dorsal spinal inhibitory interneurons is strongly implicated in the mechanisms underlying central sensitization in chronic pain. Surprisingly little is known, however, about the characteristics and consequences of Ca2+ signaling in these cells, including whether and how they are changed following a peripheral insult or injury and how such alterations might influence maladaptive pain plasticity. As a first step towards clarifying the precise role of Ca2+ signaling in dorsal spinal inhibitory neurons for central sensitization, we established methods for characterizing Ca2+ signals in genetically defined populations of these cells. In particular, we employed recombinant adeno-associated viral vectors to deliver subcellularly targeted, genetically encoded Ca2+ indicators into parvalbumin-positive spinal inhibitory neurons. Using wide-field microscopy, we observed both spontaneous and afferent fiber activity triggered Ca2+ signals in these cells. We propose that these methods may be adapted in future studies for the precise characterization and manipulation of Ca2+ signaling in diverse spinal inhibitory neuron subtypes, thereby enabling the clarification of its role in the mechanisms underlying pain chronicity and opening the door for possibly novel treatment directions.