# Image Reconstruction from Undersampled Confocal Microscopy Data using Multiresolution Based Maximum Entropy Regularization

## TL;DR

This paper introduces a multi-resolution maximum entropy regularization technique for reconstructing 2D images from undersampled confocal microscopy data, outperforming traditional total variation methods especially in noisy, low-sampling scenarios.

## Contribution

The authors develop an adaptive, multi-resolution regularization method based on maximum entropy, addressing limitations of total variation regularization in noisy, undersampled confocal microscopy data reconstruction.

## Key findings

- Proposed method outperforms total variation regularization in noisy conditions.
- Multi-resolution approach adapts to image structures at different scales.
- Demonstrated superior reconstruction quality on several examples.

## Abstract

We consider the problem of reconstructing 2D images from randomly under-sampled confocal microscopy samples. The well known and widely celebrated total variation regularization, which is the L1 norm of derivatives, turns out to be unsuitable for this problem; it is unable to handle both noise and under-sampling together. This issue is linked with the notion of phase transition phenomenon observed in compressive sensing research, which is essentially the break-down of total variation methods, when sampling density gets lower than certain threshold. The severity of this breakdown is determined by the so-called mutual incoherence between the derivative operators and measurement operator. In our problem, the mutual incoherence is low, and hence the total variation regularization gives serious artifacts in the presence of noise even when the sampling density is not very low. There has been very few attempts in developing regularization methods that perform better than total variation regularization for this problem. We develop a multi-resolution based regularization method that is adaptive to image structure. In our approach, the desired reconstruction is formulated as a series of coarse-to-fine multi-resolution reconstructions; for reconstruction at each level, the regularization is constructed to be adaptive to the image structure, where the information for adaption is obtained from the reconstruction obtained at coarser resolution level. This adaptation is achieved by using maximum entropy principle, where the required adaptive regularization is determined as the maximizer of entropy subject to the information extracted from the coarse reconstruction as constraints. We demonstrate the superiority of the proposed regularization method over existing ones using several reconstruction examples.